This study investigates the effects of chemotherapy on fertility in male osteosarcoma survivors. Osteosarcoma is a type of bone cancer, and this study aims to understand if the chemotherapy drugs cisplatin, with or without ifosfamide, affect fertility compared to males without a history of cancer. The study also examines if these drugs change sperm DNA and certain biological markers related to sperm production and hormone creation.

Participants will provide saliva, semen, and blood samples. These samples will be studied in a laboratory to check for any changes in fertility and sperm DNA. The study will look for specific biological markers to see if chemotherapy has long-term effects on fertility.

• Who can participate: Male osteosarcoma survivors who were previously enrolled in specific trials and have not experienced relapse or another cancer can participate. Participants must be able to speak, read, and write in English or Spanish.
• Study details: Participants will be asked to provide saliva, semen, and blood samples for laboratory analysis. These samples will be used to study potential changes in fertility and DNA caused by chemotherapy.